Journal: Radiation Oncology (London, England)

Article Title: Resveratrol enhances prostate cancer cell response to ionizing radiation. Modulation of the AMPK, Akt and mTOR pathways

doi: 10.1186/1748-717X-6-144

Figure Lengend Snippet: Effects of resveratrol (RSV) and ionizing radiation (IR) on survival of prostate cancer (PrCa) cells . A . Cells were incubated with increasing doses of RSV (0-10 μM) and allowed to form colonies. Colonies (> 50 cells) were counted. Results are expressed as the surviving fraction compared to untreated control (see Methods) . '*' p < 0.05 compared to untreated PC3 cells, '#' p < 0.05 compared to untreated 22RV1 cells, '+' p < 0.05 compared to untreated PNT1A cells. B . Cells were pre-treated with RSV (0-10 μM) followed by treatment with a single fraction of IR (2 Gy) and allowed to grow and form colonies. Treatment values were normalized to untreated controls and surviving fractions are presented as percent of control. Shown is the mean of 4-5 independent experiments ± standard error (SE). '*' p < 0.05 compared to 2 Gy treated PC3 cells, '#' p < 0.05 compared to 2 Gy treated 22RV1 cells, '+' p < 0.05 compared to 2 Gy treated PNT1A cells. C . Projected proportion of tumour survival after treatment with 0 - 80 Gy was estimated using the Surviving Fraction at 2 Gy (SF2) values for control and RSV (5 μM) treated PNT1A, PC3 and 22RV1 cells. SF2 n was determined as the tumour proportion surviving after "n" 2 Gy fractions, assuming iso-effectiveness of each fraction. Values were plotted up to a scale of 1 × 10 -11 to reflect elimination of tumour of 50 - 100 gr expected to contain 5 - 10 × 10 10 cells.

Article Snippet: Human PrCa (PC3, 22RV1) and normal prostate epithelial (PNT1A) cell lines were obtained from American Tissue Culture Collection (Manassas, VA, U.S.A.).

Techniques: Incubation, Control

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Control, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Expressing, Control